WASF2 Kit ELISA

N° du produit ABIN5660028

Détail du produit WASF2 Kit ELISA

Antigène: WASF2 (WAS Protein Family, Member 2 (WASF2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2). No significant cross-reactivity or interference between Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) and analogues was observed.

Sensibilité: 0.106 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: WASF2 (WAS Protein Family, Member 2 (WASF2))

Autre désignation: Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2 Produits)

Synonymes: zgc:56010 Kit ELISA, WASF2 Kit ELISA, scar2 Kit ELISA, wave2 Kit ELISA, IMD2 Kit ELISA, SCAR2 Kit ELISA, WASF4 Kit ELISA, WAVE2 Kit ELISA, dJ393P12.2 Kit ELISA, AW742646 Kit ELISA, D4Ertd13e Kit ELISA, WAS protein family, member 2 Kit ELISA, WAS protein family member 2 Kit ELISA, WAS protein family member 2 L homeolog Kit ELISA, wasf2 Kit ELISA, WASF2 Kit ELISA, wasf2.L Kit ELISA, Wasf2 Kit ELISA

Sujet:

Gene Name: Wiskott Aldrich Syndrome Protein Family, Member 2

Gene Aliases: SCAR2, WAVE2, Verprolin homology domain-containing protein 2

Pathways: Signalisation RTK