ERCC5 Kit CLIA

N° du produit ABIN5660071

Détail du produit ERCC5 Kit CLIA

Antigène: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Xeroderma Pigmentosum, Complementation Group G (XPG). No significant cross-reactivity or interference between Xeroderma Pigmentosum, Complementation Group G (XPG) and analogues was observed.

Sensibilité: 5.51 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Xeroderma Pigmentosum, Complementation Group G (XPG) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group G (XPG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group G (XPG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Autre désignation: Xeroderma Pigmentosum, Complementation Group G (ERCC5 Produits)

Synonymes: COFS3 Kit CLIA, ERCM2 Kit CLIA, UVDR Kit CLIA, XPG Kit CLIA, XPGC Kit CLIA, cofs3 Kit CLIA, ercm2 Kit CLIA, uvdr Kit CLIA, xpg Kit CLIA, xpgc Kit CLIA, Xpg Kit CLIA, ERCC excision repair 5, endonuclease Kit CLIA, excision repair cross-complementation group 5 L homeolog Kit CLIA, excision repair cross-complementing rodent repair deficiency, complementation group 5 Kit CLIA, ERCC5 Kit CLIA, ercc5.L Kit CLIA, Ercc5 Kit CLIA

Sujet:

Gene Name: Xeroderma Pigmentosum, Complementation Group G

Gene Aliases: ERCM2, ERCC5, UVDR, XPGC, Excision Repair Cross-Complementing Rodent Repair Deficiency 5, Cockayne Syndrome, DNA repair protein complementing XP-G cells

ID gène: 2073

UniProt: P28715

Pathways: Réparation de l'ADN