YY1 Kit ELISA

N° du produit ABIN5660075

Détail du produit YY1 Kit ELISA

Antigène: YY1 (YY1 Transcription Factor (YY1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of YY1 Transcription Factor (YY1). No significant cross-reactivity or interference between YY1 Transcription Factor (YY1) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to YY1 Transcription Factor (YY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to YY1 Transcription Factor (YY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain YY1 Transcription Factor (YY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of YY1 Transcription Factor (YY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level YY1 Transcription Factor (YY1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level YY1 Transcription Factor (YY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: YY1 (YY1 Transcription Factor (YY1))

Autre désignation: YY1 Transcription Factor (YY1 Produits)

Synonymes: yy1-a Kit ELISA, FIII Kit ELISA, MGC83714 Kit ELISA, yy1l Kit ELISA, zgc:64207 Kit ELISA, YY1 Kit ELISA, LOC494431 Kit ELISA, xyy1 Kit ELISA, delta Kit ELISA, nf-e1 Kit ELISA, ucrbp Kit ELISA, yin-yang-1 Kit ELISA, LOC100304682 Kit ELISA, ino80s Kit ELISA, yy1 Kit ELISA, DELTA Kit ELISA, INO80S Kit ELISA, NF-E1 Kit ELISA, UCRBP Kit ELISA, YIN-YANG-1 Kit ELISA, AW488674 Kit ELISA, fb59g10 Kit ELISA, wu:fa16g07 Kit ELISA, wu:fb59g10 Kit ELISA, YY1 transcription factor L homeolog Kit ELISA, YY1 transcription factor b Kit ELISA, YY1 transcription factor Kit ELISA, transcriptional repressor protein YY1 Kit ELISA, YY1 transcription factor S homeolog Kit ELISA, YY1 transcription factor a Kit ELISA, YY2 transcription factor Kit ELISA, yy1.L Kit ELISA, yy1b Kit ELISA, YY1 Kit ELISA, yy1 Kit ELISA, LOC100304682 Kit ELISA, yy1.S Kit ELISA, Yy1 Kit ELISA, yy1a Kit ELISA, YY2 Kit ELISA

Sujet:

Gene Name: YY1 Transcription Factor

Gene Aliases: NF-E1, DELTA, UCRBP, YIN-YANG-1, INO80S, INO80 Complex Subunit S, Yin and Yang 1 Protein, Delta transcription factor, INO80 complex subunit S