ZEB2 Kit ELISA

N° du produit ABIN5660077

Détail du produit ZEB2 Kit ELISA

Antigène: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Zinc Finger Homeobox Protein 1B (ZFHX1B). No significant cross-reactivity or interference between Zinc Finger Homeobox Protein 1B (ZFHX1B) and analogues was observed.

Sensibilité: 0.051 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zinc Finger Homeobox Protein 1B (ZFHX1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Zinc Finger Homeobox Protein 1B (ZFHX1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zinc Finger Homeobox Protein 1B (ZFHX1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zinc Finger Homeobox Protein 1B (ZFHX1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Autre désignation: Zinc Finger Homeobox Protein 1B (ZEB2 Produits)

Synonymes: ZFHX1B Kit ELISA, Zfhx1b Kit ELISA, HSPC082 Kit ELISA, SIP-1 Kit ELISA, SIP1 Kit ELISA, SMADIP1 Kit ELISA, 9130203F04Rik Kit ELISA, D130016B08Rik Kit ELISA, Zfx1b Kit ELISA, Zfxh1b Kit ELISA, mKIAA0569 Kit ELISA, XSip1 Kit ELISA, sip1 Kit ELISA, zfhx1b Kit ELISA, si:dkey-52o2.2 Kit ELISA, sip1a Kit ELISA, wu:fc92g08 Kit ELISA, sip1b Kit ELISA, zinc finger E-box binding homeobox 2 Kit ELISA, zinc finger E-box binding homeobox 2 S homeolog Kit ELISA, zinc finger homeobox protein Kit ELISA, zinc finger E-box binding homeobox 2a Kit ELISA, zinc finger E-box binding homeobox 2b Kit ELISA, ZEB2 Kit ELISA, Zeb2 Kit ELISA, zeb2.S Kit ELISA, zeb2 Kit ELISA, zeb2a Kit ELISA, zeb2b Kit ELISA

Sujet:

Gene Name: Zinc Finger Homeobox Protein 1B

Gene Aliases: ZEB2, SIP1, SIP-1, SMADIP1, SIP1, Zinc Finger E-Box Binding Homeobox 2, SMAD Interacting Protein 1

ID gène: 24136

UniProt: Q9R0G7

Pathways: Tube Formation