LDLRAP1 Kit ELISA

N° du produit ABIN5674658

Détail du produit LDLRAP1 Kit ELISA

Antigène: LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0056 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1))

Abstract: LDLRAP1 Produits

Synonymes: ARH Kit ELISA, ARH1 Kit ELISA, ARH2 Kit ELISA, FHCB1 Kit ELISA, FHCB2 Kit ELISA, AA691260 Kit ELISA, Arh Kit ELISA, Arh1 Kit ELISA, RGD1563417 Kit ELISA, arh Kit ELISA, arh1 Kit ELISA, arh2 Kit ELISA, fhcb1 Kit ELISA, fhcb2 Kit ELISA, xptb Kit ELISA, LDLRAP1 Kit ELISA, ldlrap1 Kit ELISA, sb:cb50 Kit ELISA, zgc:56121 Kit ELISA, zgc:158745 Kit ELISA, low density lipoprotein receptor adaptor protein 1 Kit ELISA, low density lipoprotein receptor adaptor protein 1 L homeolog Kit ELISA, low density lipoprotein receptor adaptor protein 1 S homeolog Kit ELISA, low density lipoprotein receptor adaptor protein 1a Kit ELISA, low density lipoprotein receptor adaptor protein 1b Kit ELISA, LDLRAP1 Kit ELISA, Ldlrap1 Kit ELISA, ldlrap1 Kit ELISA, ldlrap1.L Kit ELISA, ldlrap1.S Kit ELISA, ldlrap1a Kit ELISA, ldlrap1b Kit ELISA

Sujet: Alternative name: ARH1, ARH, ARH2, FHCB1, FHCB2, Autosomal recessive hypercholesterolemia protein

ID gène: 26119

UniProt: Q5SW96

Pathways: Lipid Metabolism