Apelin Receptor Kit ELISA
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- Antigène Voir toutes Apelin Receptor (APLNR) Kits ELISA
- Apelin Receptor (APLNR)
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.625 pg/mL - 1000 pg/mL
- Seuil minimal de détection
- 15.625 pg/mL
- Application
- ELISA
- Type d'échantillon
- Tissue Homogenate
- Analytical Method
- Quantitative
- Sensibilité
- 5.9 pg/mL
- Ingrédients
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Matériel non inclus
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
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- Antigène Voir toutes Apelin Receptor (APLNR) Kits ELISA
- Apelin Receptor (APLNR)
- Abstract
- APLNR Produits
- Synonymes
- AGTRL1 Kit ELISA, APJ Kit ELISA, APJR Kit ELISA, HG11 Kit ELISA, Agtrl1 Kit ELISA, msr/apj Kit ELISA, Apj Kit ELISA, X-msr Kit ELISA, Xangio1 Kit ELISA, Xmsr Kit ELISA, agtrl1 Kit ELISA, angio1 Kit ELISA, apj Kit ELISA, apjr Kit ELISA, hg11 Kit ELISA, msr Kit ELISA, APLNR Kit ELISA, DKFZp469C1532 Kit ELISA, agtrl1-B Kit ELISA, agtrl1b Kit ELISA, Agtrl1a Kit ELISA, grn Kit ELISA, zgc:114063 Kit ELISA, APJa Kit ELISA, XAngio1 Kit ELISA, agtrl1-A Kit ELISA, agtrl1a Kit ELISA, aplnr-a Kit ELISA, aplnr-b Kit ELISA, x-MSR Kit ELISA, xAngio1 Kit ELISA, APLNR1 Kit ELISA, apelin receptor Kit ELISA, apelin receptor S homeolog Kit ELISA, apelin receptor b Kit ELISA, apelin receptor L homeolog Kit ELISA, APLNR Kit ELISA, Aplnr Kit ELISA, aplnr Kit ELISA, aplnr.S Kit ELISA, aplnrb Kit ELISA, aplnr.L Kit ELISA
- Sujet
- Alternative name: AGTRL1, HG11, APJR, APJ, APJ Receptor, Angiotensin II Receptor-Like 1, Angiotensin receptor-like 1
- ID gène
- 23796
- UniProt
- Q9WV08
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