HRH1 Kit ELISA

N° du produit ABIN5674694

Détail du produit HRH1 Kit ELISA

Antigène: HRH1 (Histamine Receptor H1 (HRH1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0113 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: HRH1 (Histamine Receptor H1 (HRH1))

Abstract: HRH1 Produits

Synonymes: H1-R Kit ELISA, hisH1 Kit ELISA, Bphs Kit ELISA, H1R Kit ELISA, HH1R Kit ELISA, Hir Kit ELISA, HRH1 Kit ELISA, H1 Kit ELISA, Hisr Kit ELISA, histamine receptor H1 Kit ELISA, histamine receptor H 1 Kit ELISA, HRH1 Kit ELISA, Hrh1 Kit ELISA, hrh1 Kit ELISA

Sujet: Alternative name: H1-R, hisH1

ID gène: 15465

UniProt: P70174

Pathways: Regulation of Carbohydrate Metabolic Process