PLP1 Kit ELISA

N° du produit ABIN5674700

Détail du produit PLP1 Kit ELISA

Antigène: PLP1 (Proteolipid Protein 1 (PLP1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 26 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: PLP1 (Proteolipid Protein 1 (PLP1))

Autre désignation: Proteolipid Protein 1, Myelin (PLP1) (PLP1 Produits)

Synonymes: GPM6C Kit ELISA, HLD1 Kit ELISA, MMPL Kit ELISA, PLP Kit ELISA, PLP/DM20 Kit ELISA, PMD Kit ELISA, SPG2 Kit ELISA, Plp Kit ELISA, PLP1 Kit ELISA, plp1 Kit ELISA, DKFZp459O081 Kit ELISA, DKFZp459O113 Kit ELISA, DM20 Kit ELISA, jimpy Kit ELISA, jp Kit ELISA, msd Kit ELISA, rsh Kit ELISA, plp Kit ELISA, hld1 Kit ELISA, mmpl Kit ELISA, plp1a Kit ELISA, pmd Kit ELISA, spg2 Kit ELISA, DMalpha2c Kit ELISA, wu:fc27f01 Kit ELISA, wu:fj36d03 Kit ELISA, wu:fj42d08 Kit ELISA, zgc:110499 Kit ELISA, PLP-B Kit ELISA, plp1-a Kit ELISA, plp1-b Kit ELISA, plp1b Kit ELISA, proteolipid protein 1 Kit ELISA, proteolipid protein (myelin) 1 Kit ELISA, myelin proteolipid protein Kit ELISA, proteolipid protein 1 L homeolog Kit ELISA, proteolipid protein 1a Kit ELISA, proteolipid protein 1 S homeolog Kit ELISA, PLP1 Kit ELISA, Plp1 Kit ELISA, plp1 Kit ELISA, Tsp_11640 Kit ELISA, plp Kit ELISA, plp1.L Kit ELISA, plp1a Kit ELISA, plp1.S Kit ELISA

Sujet: Alternative name: MMPL, PLP/DM20, PMD, SPG2, Pelizaeus-Merzbacher Disease, Spastic Paraplegia 2,Uncomplicated, Myelin proteolipid protein, Lipophilin

ID gène: 24943

UniProt: P60203