NKAP Kit ELISA

N° du produit ABIN5674704

Détail du produit NKAP Kit ELISA

Antigène: NKAP (NFKB Activating Protein (NKAP))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 11.3 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: NKAP (NFKB Activating Protein (NKAP))

Abstract: NKAP Produits

Synonymes: DSMA4 Kit ELISA, GEF720 Kit ELISA, Syx Kit ELISA, Tech Kit ELISA, c6orf194l Kit ELISA, wu:fj36d09 Kit ELISA, wu:fj49c09 Kit ELISA, 2610020O08Rik Kit ELISA, AA987160 Kit ELISA, AI849147 Kit ELISA, AL024236 Kit ELISA, 2610020o08rik Kit ELISA, nkap Kit ELISA, NKAPL Kit ELISA, NFKB activating protein Kit ELISA, pleckstrin homology and RhoGEF domain containing G5 Kit ELISA, NFKB activating protein S homeolog Kit ELISA, NKAP Kit ELISA, PLEKHG5 Kit ELISA, nkap Kit ELISA, Nkap Kit ELISA, nkap.S Kit ELISA

Sujet: Alternative name: NF Kappa B Activating Protein

ID gène: 67050

UniProt: Q9D0F4

Pathways: Stem Cell Maintenance, Phosphorylation & l'infection par le SRAS-CoV-2