NRF1 Kit ELISA

N° du produit ABIN5674706

Détail du produit NRF1 Kit ELISA

Antigène: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 30.98 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Abstract: NRF1 Produits

Synonymes: ALPHA-PAL Kit ELISA, C87038 Kit ELISA, D6Ertd415e Kit ELISA, SC:BZ3C13.1 Kit ELISA, SC:bZ3C13.2 Kit ELISA, ube2h Kit ELISA, Nfe2l1_retired Kit ELISA, IBR/F Kit ELISA, NRF1 Kit ELISA, alpha-pal Kit ELISA, nrf-1 Kit ELISA, LOC100220087 Kit ELISA, nuclear respiratory factor 1 Kit ELISA, nuclear respiratory factor 1 S homeolog Kit ELISA, NRF1 Kit ELISA, Nrf1 Kit ELISA, nrf1 Kit ELISA, nrf1.S Kit ELISA

Sujet: Alternative name: ALPHA-PAL, EWG, Alpha Palindromic-Binding Protein

ID gène: 4899

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha