SF3B3 Kit ELISA

N° du produit ABIN5674722

Détail du produit SF3B3 Kit ELISA

Antigène: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0065 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Autre désignation: Splicing Factor 3B Subunit 3 (SF3B3) (SF3B3 Produits)

Synonymes: GB11574 Kit ELISA, rse1 Kit ELISA, sap130 Kit ELISA, sf3b130 Kit ELISA, staf130 Kit ELISA, sf3b3 Kit ELISA, 1810061H24Rik Kit ELISA, 5730409A01Rik Kit ELISA, AA409318 Kit ELISA, D8Ertd633e Kit ELISA, RSE1 Kit ELISA, SAP130 Kit ELISA, mKIAA0017 Kit ELISA, ik:tdsubc_2b2 Kit ELISA, wu:fb81f05 Kit ELISA, xx:tdsubc_2b2 Kit ELISA, zgc:55440 Kit ELISA, SF3b130 Kit ELISA, STAF130 Kit ELISA, splicing factor 3b subunit 3 Kit ELISA, splicing factor 3B subunit 3 Kit ELISA, splicing factor 3b, subunit 3, 130kDa Kit ELISA, splicing factor 3b subunit 3 L homeolog Kit ELISA, splicing factor 3b, subunit 3 Kit ELISA, SF3B3 Kit ELISA, LOC550935 Kit ELISA, sf3b3 Kit ELISA, Sf3b3 Kit ELISA, LOC100178056 Kit ELISA, sf3b3.L Kit ELISA

Sujet: Alternative name: RSE1, SAP130, SF3b130, STAF130, Spliceosome-associated protein 130, Pre-mRNA-splicing factor SF3b 130 kDa subunit

ID gène: 23450

UniProt: Q15393