SOCS1 Kit ELISA

N° du produit ABIN5674725

Détail du produit SOCS1 Kit ELISA

Antigène: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0.14 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Autre désignation: Suppressors Of Cytokine Signaling 1 (SOCS1) (SOCS1 Produits)

Synonymes: socs1 Kit ELISA, zgc:91868 Kit ELISA, jab Kit ELISA, cis1 Kit ELISA, ssi1 Kit ELISA, tip3 Kit ELISA, cish1 Kit ELISA, ssi-1 Kit ELISA, socs-1 Kit ELISA, SOCS1 Kit ELISA, LOC100228306 Kit ELISA, socs1b Kit ELISA, CIS1 Kit ELISA, CISH1 Kit ELISA, JAB Kit ELISA, SOCS-1 Kit ELISA, SSI-1 Kit ELISA, SSI1 Kit ELISA, TIP3 Kit ELISA, Cish1 Kit ELISA, Cish7 Kit ELISA, Socs-1 Kit ELISA, suppressor of cytokine signaling 1a Kit ELISA, suppressor of cytokine signaling 1 Kit ELISA, suppressor of cytokine signaling 1 L homeolog Kit ELISA, socs1a Kit ELISA, socs1 Kit ELISA, SOCS1 Kit ELISA, socs1.L Kit ELISA, Socs1 Kit ELISA

Sujet: Alternative name: CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP3, JAK-binding protein, STAT-induced STAT inhibitor 1, Tec-interacting protein 3

ID gène: 12703

UniProt: O35716

Pathways: Signalistation JAK/STAT, Interferon-gamma Pathway, Signalisation TLR, Response to Growth Hormone Stimulus