Peripherin Kit ELISA
PRPH
Reactivité: Humain
Colorimetric
Sandwich ELISA
78.125 pg/mL - 5000 pg/mL
Cell Lysate, Tissue Homogenate
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- Antigène Voir toutes Peripherin (PRPH) Kits ELISA
- Peripherin (PRPH)
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 78.125 pg/mL - 5000 pg/mL
- Seuil minimal de détection
- 78.125 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensibilité
- 32.7 pg/mL
- Ingrédients
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Matériel non inclus
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product PRPH Kit ELISA
-
Peripherin (PRPH) ELISA Kit
PRPH Reactivité: Souris Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
-
- Antigène Voir toutes Peripherin (PRPH) Kits ELISA
- Peripherin (PRPH)
- Abstract
- PRPH Produits
- Synonymes
- NEF4 Kit ELISA, PRPH1 Kit ELISA, Prph1 Kit ELISA, Perf Kit ELISA, nef4 Kit ELISA, prph1 Kit ELISA, MGC69454 Kit ELISA, if3 Kit ELISA, plasticin Kit ELISA, zgc:111926 Kit ELISA, peripherin Kit ELISA, peripherin L homeolog Kit ELISA, PRPH Kit ELISA, Prph Kit ELISA, prph Kit ELISA, prph.L Kit ELISA
- Sujet
- Alternative name: NEF4, Neurofilament 4
- ID gène
- 5630
- UniProt
- P41219
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