APEX1 Kit ELISA

N° du produit ABIN5674748

Détail du produit APEX1 Kit ELISA

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0143 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Autre désignation: APEX Nuclease 1 (APEX1) (APEX1 Produits)

Synonymes: apex1 Kit ELISA, MGC76280 Kit ELISA, MGC79645 Kit ELISA, MGC78928 Kit ELISA, APE Kit ELISA, APE1 Kit ELISA, APEN Kit ELISA, APEX Kit ELISA, APX Kit ELISA, HAP1 Kit ELISA, REF1 Kit ELISA, Apex Kit ELISA, REF-1 Kit ELISA, BAP1 Kit ELISA, apex1a Kit ELISA, hm:zehn1532 Kit ELISA, wu:fc13d11 Kit ELISA, zgc:66204 Kit ELISA, Ref-1 Kit ELISA, apurinic/apyrimidinic endodeoxyribonuclease 1 Kit ELISA, APEX nuclease (multifunctional DNA repair enzyme) 1 Kit ELISA, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog Kit ELISA, DNA-(apurinic or apyrimidinic site) lyase Kit ELISA, apurinic/apyrimidinic endonuclease 1 Kit ELISA, APEX1 Kit ELISA, apex1 Kit ELISA, apex1.L Kit ELISA, CNG03640 Kit ELISA, CNH01280 Kit ELISA, nth Kit ELISA, Spirs_4119 Kit ELISA, Palpr_0103 Kit ELISA, Apex1 Kit ELISA

Sujet: Alternative name: APE, APE1, APEN, APEX, APX, HAP1, REF1, Multifunctional DNA Repair Enzyme, APEX Nuclease 1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase

ID gène: 79116

UniProt: P43138

Pathways: Réparation de l'ADN, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus