ARHGEF7 Kit ELISA

N° du produit ABIN5674758

Détail du produit ARHGEF7 Kit ELISA

Antigène: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 34 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Autre désignation: Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) (ARHGEF7 Produits)

Synonymes: BETA-PIX Kit ELISA, COOL-1 Kit ELISA, COOL1 Kit ELISA, Nbla10314 Kit ELISA, P50 Kit ELISA, P50BP Kit ELISA, P85 Kit ELISA, P85COOL1 Kit ELISA, P85SPR Kit ELISA, PAK3 Kit ELISA, PIXB Kit ELISA, cool1 Kit ELISA, p50 Kit ELISA, p85 Kit ELISA, pak3 Kit ELISA, pixb Kit ELISA, ARHGEF7 Kit ELISA, P85spr Kit ELISA, Pak3bp Kit ELISA, Cool Kit ELISA, PIX Kit ELISA, betaPix Kit ELISA, betaPix-b Kit ELISA, betaPix-c Kit ELISA, cool-1 Kit ELISA, mKIAA0142 Kit ELISA, p85Cool1 Kit ELISA, p85SPR Kit ELISA, arhgef7 Kit ELISA, bbh Kit ELISA, bpix Kit ELISA, im:7142367 Kit ELISA, wu:fc35h08 Kit ELISA, zgc:101775 Kit ELISA, Rho guanine nucleotide exchange factor 7 Kit ELISA, Rho guanine nucleotide exchange factor 7 S homeolog Kit ELISA, ankyrin repeat domain 10 Kit ELISA, Rho guanine nucleotide exchange factor (GEF7) Kit ELISA, Rho guanine nucleotide exchange factor (GEF) 7b Kit ELISA, ARHGEF7 Kit ELISA, arhgef7.S Kit ELISA, arhgef7 Kit ELISA, ANKRD10 Kit ELISA, Arhgef7 Kit ELISA, arhgef7b Kit ELISA

Sujet: Alternative name: PAK3, BETA-PIX, COOL1, P50, P50BP, P85, P85SPR, PIXB, SH3 Domain-Containing Proline-Rich Protein, PAK-Interacting Exchange Factor Beta

ID gène: 54126

UniProt: Q9ES28

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation