TSH receptor Kit ELISA

N° du produit ABIN5674760

Détail du produit TSH receptor Kit ELISA

Antigène: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 25.4 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Abstract: TSHR Produits

Synonymes: CHNG1 Kit ELISA, LGR3 Kit ELISA, hTSHR-I Kit ELISA, AI481368 Kit ELISA, hypothroid Kit ELISA, hyt Kit ELISA, pet Kit ELISA, TSHRA Kit ELISA, TSH-R Kit ELISA, thyroid stimulating hormone receptor Kit ELISA, tshr Kit ELISA, TSHR Kit ELISA, Tshr Kit ELISA

Sujet: Alternative name: LGR3, HTSHR-I, Thyrotropin Receptor, TSH Receptor

ID gène: 7253

UniProt: P16473

Pathways: Thyroid Hormone Synthesis