CCR2 Kit ELISA
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- Antigène Voir toutes CCR2 Kits ELISA
- CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensibilité
- 0061 ng/mL
- Ingrédients
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Matériel non inclus
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
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- Antigène Voir toutes CCR2 Kits ELISA
- CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))
- Autre désignation
- Chemokine C-C-Motif Receptor 2 (CCR2) (CCR2 Produits)
- Synonymes
- CC-CKR-2 Kit ELISA, CCR-2 Kit ELISA, CCR2A Kit ELISA, CCR2B Kit ELISA, CD192 Kit ELISA, CKR2 Kit ELISA, CKR2A Kit ELISA, CKR2B Kit ELISA, CMKBR2 Kit ELISA, MCP-1-R Kit ELISA, ACKR5 Kit ELISA, CKRX Kit ELISA, CRAM Kit ELISA, CRAM-A Kit ELISA, CRAM-B Kit ELISA, HCR Kit ELISA, Cc-ckr-2 Kit ELISA, Ccr2a Kit ELISA, Ccr2b Kit ELISA, Ckr2 Kit ELISA, Ckr2a Kit ELISA, Ckr2b Kit ELISA, Cmkbr2 Kit ELISA, mJe-r Kit ELISA, C-C motif chemokine receptor 2 Kit ELISA, C-C motif chemokine receptor like 2 Kit ELISA, chemokine (C-C motif) receptor 2 Kit ELISA, CCR2 Kit ELISA, CCRL2 Kit ELISA, Ccr2 Kit ELISA, LOC484790 Kit ELISA
- Sujet
- Alternative name: CD192, CC-CKR2, CCR2A, C-C CKR-2, CCR2B, CKR2-A, CKR2-B, CMKBR2, MCP1-R, Monocyte chemoattractant protein 1 receptor
- ID gène
- 12772
- UniProt
- P51683
- Pathways
- cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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