P4HA1 Kit ELISA

N° du produit ABIN5674784

Détail du produit P4HA1 Kit ELISA

Antigène: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0108 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Autre désignation: Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) (P4HA1 Produits)

Synonymes: P4HA Kit ELISA, AL022634 Kit ELISA, P4ha Kit ELISA, PHalphaI Kit ELISA, prolyl 4-hydroxylase subunit alpha-1 Kit ELISA, prolyl 4-hydroxylase subunit alpha 1 Kit ELISA, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide Kit ELISA, CpipJ_CPIJ000975 Kit ELISA, CpipJ_CPIJ017131 Kit ELISA, Tsp_03346 Kit ELISA, Tsp_03347 Kit ELISA, Tsp_15957 Kit ELISA, P4HA1 Kit ELISA, P4ha1 Kit ELISA

Sujet: Alternative name: Procollagen-Proline,2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase),Alpha Polypeptide I

ID gène: 18451

UniProt: Q60715