KIF5B Kit ELISA

N° du produit ABIN5674789

Détail du produit KIF5B Kit ELISA

Antigène: KIF5B (Kinesin Family Member 5B (KIF5B))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 29.4 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: KIF5B (Kinesin Family Member 5B (KIF5B))

Autre désignation: Kinesin Family, Member 5B (KIF5B) (KIF5B Produits)

Synonymes: KINH Kit ELISA, KNS Kit ELISA, KNS1 Kit ELISA, UKHC Kit ELISA, Khc Kit ELISA, AL022807 Kit ELISA, Khcs Kit ELISA, Kns1 Kit ELISA, Ukhc Kit ELISA, khc Kit ELISA, KIF5A Kit ELISA, kinesin family member 5B Kit ELISA, kinesin heavy chain Kit ELISA, kinesin-1 Kit ELISA, kinesin-1 heavy chain Kit ELISA, kinesin family member 5B S homeolog Kit ELISA, KIF5B Kit ELISA, LOC411769 Kit ELISA, cgd6_2790 Kit ELISA, kin-1 Kit ELISA, Tb927.1.1350 Kit ELISA, LACBIDRAFT_305999 Kit ELISA, Khc Kit ELISA, LOC100538629 Kit ELISA, Kif5b Kit ELISA, kif5b.S Kit ELISA

Sujet: Alternative name: KNS1, UKHC, KINH, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, Kinesin-1 heavy chain

ID gène: 16573

UniProt: Q61768

Pathways: Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization