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NRN1 Kit ELISA

NRN1 Reactivité: Humain Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5674796
  • Antigène Voir toutes NRN1 Kits ELISA
    NRN1 (Neuritin 1 (NRN1))
    Reactivité
    • 4
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    11.5 pg/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes NRN1 Kits ELISA
    NRN1 (Neuritin 1 (NRN1))
    Abstract
    NRN1 Produits
    Synonymes
    NRN Kit ELISA, dJ380B8.2 Kit ELISA, 0710008J23Rik Kit ELISA, Cpg15 Kit ELISA, Nrn Kit ELISA, neuritin Kit ELISA, nrn1 Kit ELISA, zgc:92828 Kit ELISA, NRN1 Kit ELISA, cpg15 Kit ELISA, nrn Kit ELISA, nrn1-a Kit ELISA, nrn1-b Kit ELISA, nrn1b Kit ELISA, neuritin 1 Kit ELISA, neuritin 1a Kit ELISA, neuritin 1 S homeolog Kit ELISA, neuritin 1 L homeolog Kit ELISA, NRN1 Kit ELISA, Nrn1 Kit ELISA, nrn1a Kit ELISA, nrn1 Kit ELISA, nrn1.S Kit ELISA, nrn1.L Kit ELISA
    ID gène
    51299
    UniProt
    Q9NPD7
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