HTR2C Kit ELISA

N° du produit ABIN5674799

Détail du produit HTR2C Kit ELISA

Antigène: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0064 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Autre désignation: 5-Hydroxytryptamine Receptor 2C (HTR2C) (HTR2C Produits)

Synonymes: 5-HT2C Kit ELISA, 5-HTR2C Kit ELISA, HTR1C Kit ELISA, 5-HT2cR Kit ELISA, 5HT1c Kit ELISA, Htr1c Kit ELISA, SR1 Kit ELISA, 5HT-1C Kit ELISA, 5HT2C Kit ELISA, 5-HT-2C Kit ELISA, 5-ht2cr Kit ELISA, si:dkey-24g18.2 Kit ELISA, 5ht2c Kit ELISA, 5HTR2C Kit ELISA, 5-hydroxytryptamine receptor 2C Kit ELISA, 5-hydroxytryptamine (serotonin) receptor 2C Kit ELISA, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled S homeolog Kit ELISA, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled-like 1 Kit ELISA, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled Kit ELISA, HTR2C Kit ELISA, Htr2c Kit ELISA, htr2c.S Kit ELISA, htr2cl1 Kit ELISA, LOC407134 Kit ELISA

Sujet: Alternative name: 5-HT2C, HTR1C, Serotonin Receptor 2C, 5-hydroxytryptamine receptor 1C

ID gène: 3358

UniProt: P28335

Pathways: Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour