ALAS1 Kit ELISA

N° du produit ABIN5674802

Détail du produit ALAS1 Kit ELISA

Antigène: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0131 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Autre désignation: Aminolevulinate Delta Synthase 1 (ALAS1) (ALAS1 Produits)

Synonymes: ALAS Kit ELISA, ALAS3 Kit ELISA, ALASH Kit ELISA, MIG4 Kit ELISA, ALAS-N Kit ELISA, Alas-1 Kit ELISA, Alas-h Kit ELISA, ALAS-H Kit ELISA, ALASN Kit ELISA, ALAS1 Kit ELISA, wu:fb58d01 Kit ELISA, wu:fi12g09 Kit ELISA, 5'-aminolevulinate synthase 1 Kit ELISA, aminolevulinic acid synthase 1 Kit ELISA, alanyl-tRNA synthetase protein Kit ELISA, aminolevulinate, delta-, synthase 1 Kit ELISA, ALAS1 Kit ELISA, Alas1 Kit ELISA, alas1 Kit ELISA, alas1.S Kit ELISA, alaS1 Kit ELISA

Sujet: Alternative name: ALAS3, ALAS, ALASH, 5-aminolevulinic acid synthase 1, Delta-ALA synthase 1, 5-aminolevulinate synthase, nonspecific, mitochondrial

ID gène: 211

UniProt: P13196

Pathways: Regulation of Lipid Metabolism by PPARalpha