LRP5 Kit ELISA

N° du produit ABIN5674832

Détail du produit LRP5 Kit ELISA

Antigène: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0118 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Autre désignation: Low Density Lipoprotein Receptor Related Protein 5 (LRP5) (LRP5 Produits)

Synonymes: Xlrp5 Kit ELISA, bmnd1 Kit ELISA, evr1 Kit ELISA, evr4 Kit ELISA, hbm Kit ELISA, lr3 Kit ELISA, lrp5-A Kit ELISA, lrp7 Kit ELISA, oppg Kit ELISA, ops Kit ELISA, opta1 Kit ELISA, vbch2 Kit ELISA, LRP5 Kit ELISA, LRP6 Kit ELISA, BMND1 Kit ELISA, EVR1 Kit ELISA, EVR4 Kit ELISA, HBM Kit ELISA, LR3 Kit ELISA, LRP7 Kit ELISA, OPPG Kit ELISA, OPS Kit ELISA, OPTA1 Kit ELISA, VBCH2 Kit ELISA, mKIAA4142 Kit ELISA, low-density lipoprotein receptor-related protein 5 Kit ELISA, LDL receptor related protein 5 Kit ELISA, LDL receptor related protein 5 L homeolog Kit ELISA, low density lipoprotein receptor-related protein 5 Kit ELISA, LDL receptor related protein 5 like Kit ELISA, LOC100147353 Kit ELISA, LRP5 Kit ELISA, lrp5.L Kit ELISA, lrp5 Kit ELISA, Lrp5 Kit ELISA, LRP5L Kit ELISA

Sujet: Alternative name: HBM, BMND1, EVR1, EVR4, LR3, LRP7, OPPG, OPS, OPTA1, VBCH2, Osteoporosis Pseudoglioma Syndrome, Exudative Vitreoretinopathy 1

ID gène: 4041

UniProt: O75197

Pathways: Signalisation WNT, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation