DSTYK Kit ELISA

N° du produit ABIN5674835

Détail du produit DSTYK Kit ELISA

Antigène: DSTYK (Dual serine/threonine and tyrosine Protein Kinase (DSTYK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0055 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: DSTYK (Dual serine/threonine and tyrosine Protein Kinase (DSTYK))

Abstract: DSTYK Produits

Synonymes: CAKUT1 Kit ELISA, DustyPK Kit ELISA, RIP5 Kit ELISA, RIPK5 Kit ELISA, A930019K20Rik Kit ELISA, C430014H23Rik Kit ELISA, C820013G01 Kit ELISA, Ripk5 Kit ELISA, ripk5 Kit ELISA, zgc:136421 Kit ELISA, DSTYK Kit ELISA, GB11994 Kit ELISA, DUSTYPK Kit ELISA, dual serine/threonine and tyrosine protein kinase Kit ELISA, receptor interacting protein kinase 5 Kit ELISA, dual serine/threonine and tyrosine protein kinase S homeolog Kit ELISA, DSTYK Kit ELISA, Dstyk Kit ELISA, dstyk Kit ELISA, Ripk5 Kit ELISA, dstyk.S Kit ELISA

Sujet: Alternative name: RIPK5, Dusty PK, RIP5, SgK496, Receptor Interacting Protein Kinase 5, RIP-homologous kinase, Sugen kinase 496, Receptor-interacting serine/threonine-protein kinase 5

ID gène: 25778

UniProt: Q6XUX3