PAOX Kit ELISA

N° du produit ABIN5674857

Détail du produit PAOX Kit ELISA

Antigène: PAOX (Polyamine Oxidase (Exo-N4-Amino) (PAOX))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 31.5 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: PAOX (Polyamine Oxidase (Exo-N4-Amino) (PAOX))

Autre désignation: Polyamine Oxidase (PAOX) (PAOX Produits)

Synonymes: PAO Kit ELISA, mpao1 Kit ELISA, 2410012F02Rik Kit ELISA, AI118225 Kit ELISA, Pao Kit ELISA, pao Kit ELISA, polyamine oxidase Kit ELISA, polyamine oxidase 1 Kit ELISA, amine oxidase family protein Kit ELISA, si:dkey-275b16.2 Kit ELISA, polyamine oxidase (exo-N4-amino) Kit ELISA, polyamine oxidase (exo-N4-amino) L homeolog Kit ELISA, PAOX Kit ELISA, pao1 Kit ELISA, NFIA_077590 Kit ELISA, POPTR_0011s12590g Kit ELISA, si:dkey-275b16.2 Kit ELISA, Paox Kit ELISA, paox.L Kit ELISA

Sujet: Alternative name: PAO, Peroxisomal N(1)-Acetyl-Spermine/Spermidine Oxidase(Exo-N4-Amino)

ID gène: 196743

UniProt: Q6QHF9