ACAT1 Kit ELISA

N° du produit ABIN5674861

Détail du produit ACAT1 Kit ELISA

Antigène: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 12.5 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Autre désignation: Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) (ACAT1 Produits)

Synonymes: ACAT Kit ELISA, MAT Kit ELISA, T2 Kit ELISA, THIL Kit ELISA, RATACAL Kit ELISA, 6330585C21Rik Kit ELISA, Acat Kit ELISA, fd16h07 Kit ELISA, fd20g06 Kit ELISA, wu:fd16h07 Kit ELISA, wu:fd20g06 Kit ELISA, zgc:86832 Kit ELISA, acat1-a Kit ELISA, acetyl-CoA acetyltransferase 1 Kit ELISA, acetyl-Coenzyme A acetyltransferase 1 Kit ELISA, acetyl-CoA acetyltransferase 1 L homeolog Kit ELISA, ACAT1 Kit ELISA, Acat1 Kit ELISA, acat1 Kit ELISA, acat1.L Kit ELISA

Sujet: Alternative name: THIL, ACAT, T2, MAT, Acetoacetyl Coenzyme A Thiolase

ID gène: 25014

UniProt: P17764