NEIL3 Kit ELISA

N° du produit ABIN5674878

Détail du produit NEIL3 Kit ELISA

Antigène: NEIL3 (Nei Endonuclease VIII-Like 3 (NEIL3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 34.55 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: NEIL3 (Nei Endonuclease VIII-Like 3 (NEIL3))

Autre désignation: Nei Endonuclease VIII Like Protein 3 (NEIL3) (NEIL3 Produits)

Synonymes: RGD1310562 Kit ELISA, NEIL3 Kit ELISA, zgc:103553 Kit ELISA, neil3 Kit ELISA, MAC12.12 Kit ELISA, MAC12_12 Kit ELISA, FGP2 Kit ELISA, FPG2 Kit ELISA, NEI3 Kit ELISA, hFPG2 Kit ELISA, hNEI3 Kit ELISA, AI449477 Kit ELISA, BC034753 Kit ELISA, C85903 Kit ELISA, nei-like DNA glycosylase 3 Kit ELISA, nei like DNA glycosylase 3 Kit ELISA, GRF zinc finger / Zinc knuckle protein Kit ELISA, nei-like DNA glycosylase 3 L homeolog Kit ELISA, nei like 3 (E. coli) Kit ELISA, Neil3 Kit ELISA, NEIL3 Kit ELISA, neil3 Kit ELISA, AT5G13920 Kit ELISA, neil3.L Kit ELISA

Sujet: Alternative name: FPG2, hFPG2, hNEI3, Endonuclease 8-like 3, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Nei-like protein 3

ID gène: 55247

UniProt: Q8TAT5

Pathways: Réparation de l'ADN