JAK2 Kit ELISA
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- JAK2 (Janus Kinase 2 (JAK2))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 78.125 pg/mL - 5000 pg/mL
- Seuil minimal de détection
- 78.125 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensibilité
- 33.5 pg/mL
- Ingrédients
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Matériel non inclus
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product JAK2 Kit ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
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- Antigène Voir toutes JAK2 Kits ELISA
- JAK2 (Janus Kinase 2 (JAK2))
- Abstract
- JAK2 Produits
- Synonymes
- JAK2bCA Kit ELISA, jak2 Kit ELISA, JTK10 Kit ELISA, THCYT3 Kit ELISA, AI504024 Kit ELISA, C81284 Kit ELISA, Fd17 Kit ELISA, JAK-2 Kit ELISA, Janus kinase 2b Kit ELISA, Janus kinase 2 Kit ELISA, jak2b Kit ELISA, JAK2 Kit ELISA, Jak2 Kit ELISA
- Sujet
- Alternative name: JTK10, Tyrosine-protein kinase JAK2
- ID gène
- 3717
- UniProt
- O60674
- Pathways
- Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response
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