PDE3A Kit ELISA

N° du produit ABIN5674894

Détail du produit PDE3A Kit ELISA

Antigène: PDE3A (phosphodiesterase 3A, cGMP-Inhibited (PDE3A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0101 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: PDE3A (phosphodiesterase 3A, cGMP-Inhibited (PDE3A))

Autre désignation: Phosphodiesterase 3A, cGMP Inhibited (PDE3A) (PDE3A Produits)

Synonymes: PDE3A Kit ELISA, Pde3a Kit ELISA, si:dkey-48h7.2 Kit ELISA, A930022O17Rik Kit ELISA, C87899 Kit ELISA, RNPDE3A Kit ELISA, CGI-PDE Kit ELISA, CGI-PDE A Kit ELISA, CGI-PDE-A Kit ELISA, phosphodiesterase 3A Kit ELISA, phosphodiesterase 3A, cGMP-inhibited Kit ELISA, cGMP-inhibited 3',5'-cyclic phosphodiesterase A Kit ELISA, phosphodiesterase 3A, cGMP inhibited Kit ELISA, PDE3A Kit ELISA, Pde3a Kit ELISA, LOC100540515 Kit ELISA, pde3a Kit ELISA

Sujet: Alternative name: CGI-PDE, Cyclic GMP-inhibited phosphodiesterase A, cGMP-inhibited 3',5'-cyclic phosphodiesterase A

ID gène: 5139

UniProt: Q14432

Pathways: cAMP Metabolic Process