ChT Kit ELISA

N° du produit ABIN5674901

Détail du produit ChT Kit ELISA

Antigène: ChT (High Affinity Choline Transporter (ChT))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0055 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ChT (High Affinity Choline Transporter (ChT))

Abstract: ChT Produits

Synonymes: CHT Kit ELISA, CHT1 Kit ELISA, HMN7A Kit ELISA, hCHT Kit ELISA, Cht1 Kit ELISA, solute carrier family 5 member 7 Kit ELISA, solute carrier family 5 (choline transporter), member 7 Kit ELISA, SLC5A7 Kit ELISA, Slc5a7 Kit ELISA

Sujet: Alternative name: SLC5A7, hCHT, CHT1, Solute Carrier Family 5 Member 7,Choline Transporter, Hemicholinium-3-sensitive choline transporter

ID gène: 63993

UniProt: Q8BGY9