Transglutaminase 2 Kit ELISA

N° du produit ABIN5674907

Détail du produit Transglutaminase 2 Kit ELISA

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0058 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Autre désignation: Transglutaminase 2, Tissue (TGM2) (TGM2 Produits)

Synonymes: G-ALPHA-h Kit ELISA, GNAH Kit ELISA, TG2 Kit ELISA, TGC Kit ELISA, tgm2 Kit ELISA, zgc:101081 Kit ELISA, TgaseII Kit ELISA, TGM2 Kit ELISA, fc12b04 Kit ELISA, wu:fc12b04 Kit ELISA, zgc:136700 Kit ELISA, TGL2 Kit ELISA, G[a]h Kit ELISA, TGase2 Kit ELISA, tTG Kit ELISA, tTGas Kit ELISA, transglutaminase 2 Kit ELISA, transglutaminase 2, C polypeptide A Kit ELISA, transglutaminase 2b Kit ELISA, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) Kit ELISA, transglutaminase 2, C polypeptide Kit ELISA, TGM2 Kit ELISA, tgm2a Kit ELISA, Tgm2 Kit ELISA, tgm2b Kit ELISA

Sujet: Alternative name: tTG, TG2, C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase, Transglutaminase C, Transglutaminase H

ID gène: 21817

UniProt: P21981

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling