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NME1 Kit ELISA

NME1 Reactivité: Souris Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
N° du produit ABIN5674914
  • Antigène Voir toutes NME1 Kits ELISA
    NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
    Reactivité
    • 3
    • 2
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Sensibilité
    0053 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes NME1 Kits ELISA
    NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
    Autre désignation
    Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) (NME1 Produits)
    Synonymes
    AWD Kit ELISA, GAAD Kit ELISA, NB Kit ELISA, NBS Kit ELISA, NDKA Kit ELISA, NDPK-A Kit ELISA, NDPKA Kit ELISA, NM23 Kit ELISA, NM23-H1 Kit ELISA, AL024257 Kit ELISA, NM23-M1 Kit ELISA, NM23A Kit ELISA, NDPK-Z1 Kit ELISA, NM23-B Kit ELISA, NM23-Z1 Kit ELISA, ndpkz1 Kit ELISA, nm23b Kit ELISA, nme1 Kit ELISA, nme2 Kit ELISA, nme2b1 Kit ELISA, NME1 Kit ELISA, NME1-NME2 Kit ELISA, NM23-C1 Kit ELISA, ndpk-b Kit ELISA, ndpkb Kit ELISA, nm23-h2 Kit ELISA, nm23ndk Kit ELISA, puf Kit ELISA, NBR-A Kit ELISA, NBR-B Kit ELISA, NME1-1 Kit ELISA, nm23ndk-a Kit ELISA, NDK I Kit ELISA, NDPK I Kit ELISA, T30A10.80 Kit ELISA, T30A10_80 Kit ELISA, NDP kinase I Kit ELISA, NME/NM23 nucleoside diphosphate kinase 1 Kit ELISA, NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 Kit ELISA, non-metastatic cells 1, protein (NM23A) expressed in Kit ELISA, NME/NM23 nucleoside diphosphate kinase 2 S homeolog Kit ELISA, NME/NM23 nucleoside diphosphate kinase 2 L homeolog Kit ELISA, nucleoside diphosphate kinase Kit ELISA, nucleoside diphosphate kinase 1 Kit ELISA, NME1 Kit ELISA, Nme1 Kit ELISA, nme2b.1 Kit ELISA, nme2.S Kit ELISA, nme2.L Kit ELISA, LOC547870 Kit ELISA, NDPK1 Kit ELISA, LOC107790563 Kit ELISA
    Sujet
    Alternative name: AWD, GAAD, NDPKA, NM23-H1, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein
    ID gène
    18102
    UniProt
    P15532
    Pathways
    Apoptose, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process
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