LKB1 Kit ELISA

N° du produit ABIN5674931

Détail du produit LKB1 Kit ELISA

Antigène: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0057 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Abstract: STK11 Produits

Synonymes: pjs Kit ELISA, LKB1 Kit ELISA, XEEK1 Kit ELISA, Stk11 Kit ELISA, PJS Kit ELISA, hLKB1 Kit ELISA, AA408040 Kit ELISA, Lkb1 Kit ELISA, Par-4 Kit ELISA, R75140 Kit ELISA, mLKB1 Kit ELISA, wu:fj61a05 Kit ELISA, zgc:110180 Kit ELISA, xeek1 Kit ELISA, serine/threonine kinase 11 Kit ELISA, polarization-related protein LKB1 Kit ELISA, serine/threonine kinase 11 L homeolog Kit ELISA, STK11 Kit ELISA, stk11 Kit ELISA, LOC662493 Kit ELISA, Stk11 Kit ELISA, stk11.L Kit ELISA

Sujet: Alternative name: LKB1, PJS, Polarization-Related Protein LKB1, Peutz-Jeghers Syndrome, Liver kinase B1, Renal carcinoma antigen NY-REN-19

UniProt: D4AE59

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg