SESN1 Kit ELISA
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- Antigène Voir toutes SESN1 Kits ELISA
- SESN1 (Sestrin 1 (SESN1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Tissue Homogenate
- Analytical Method
- Quantitative
- Sensibilité
- 0064 ng/mL
- Ingrédients
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Matériel non inclus
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
-
- Antigène Voir toutes SESN1 Kits ELISA
- SESN1 (Sestrin 1 (SESN1))
- Abstract
- SESN1 Produits
- Synonymes
- zgc:91970 Kit ELISA, sestrin-1 Kit ELISA, SESN1 Kit ELISA, XPA26 Kit ELISA, pa26 Kit ELISA, sest1 Kit ELISA, PA26 Kit ELISA, SEST1 Kit ELISA, 1110002G11Rik Kit ELISA, AU044290 Kit ELISA, Pa26 Kit ELISA, Sest1 Kit ELISA, sesn1-A Kit ELISA, sestrin 1 Kit ELISA, sestrin 1 L homeolog Kit ELISA, SESN1 Kit ELISA, sesn1 Kit ELISA, Sesn1 Kit ELISA, sesn1.L Kit ELISA
- Sujet
- Alternative name: SEST1, PA26, p53-regulated protein PA26
- ID gène
- 27244
- UniProt
- Q9Y6P5
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