JKAMP Kit ELISA

N° du produit ABIN5674944

Détail du produit JKAMP Kit ELISA

Antigène: JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0.35 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))

Autre désignation: JNK1/MAPK8 Associated Membrane Protein (JKAMP) (JKAMP Produits)

Synonymes: C14orf100 Kit ELISA, HSPC213 Kit ELISA, HSPC327 Kit ELISA, JAMP Kit ELISA, 1200003C05Rik Kit ELISA, 2310047L11Rik Kit ELISA, Jamp Kit ELISA, RGD1308917 Kit ELISA, JNK1/MAPK8 associated membrane protein Kit ELISA, JNK1/MAPK8-associated membrane protein Kit ELISA, JKAMP Kit ELISA, Jkamp Kit ELISA

Sujet: Alternative name: JAMP, C14orf100, Jun N-Terminal Kinase 1-Associated Membrane Protein, Medulloblastoma antigen MU-MB-50.4

ID gène: 51528

UniProt: Q9P055