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c-MET Kit IQ-ELISA

MET Reactivité: Humain qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN5680031
  • Antigène Voir toutes c-MET (MET) IQ-ELISA Kits
    c-MET (MET) (Met Proto-Oncogene (MET))
    Reactivité
    • 12
    • 9
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    qPCR
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Immunoquantitative (PCR-Based) HGFR ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Semi-Quantitative
    Sensibilité
    6 pg/mL
    Attributs du produit
    The Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA) is an innovative assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The Human HGFR IQELISA kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of human HGFR in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human HGFR coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and HGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.
    Ingrédients
    • HGFR Microplate (Item A): 96 well PCR plate coated with anti-human HGFR
    • Wash Buffer I Concentrate (20x) (Item B): 20x concentrated solution
    • Standards (Item C): recombinant human HGFR
    • Assay Diluent A (Item D): diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
    • Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
    • Detection Affinity Reagent for HGFR (Item F): 4x concentrated solution of anti-human HGFR affinity reagent
    • Primer Solution (Item G)
    • PCR Master Mix (Item H)
    • PCR Preparation buffer (Item I): 10x concentrated buffer
    • Final Wash Buffer (Item J): 10x concentrated buffer
    Matériel non inclus
    • Real-time PCR instrument, Bio-Rad recommended
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
    • Log-log graph paper or computer and software for data analysis
    • Tubes to prepare standard or sample dilutions
    • Heating block or water bath capable of 80 °C
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  • Indications d'application
    The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
    Commentaires

    Features:
    - Excellent for follow up/confirmation of antibody array data
    - Pre-coated 96 well plate
    - Strip plate format minimizes waste
    - Complete kit includes all necessary reagents
    - 10x more sensitive than ELISA
    - Reproducible and reliable
    - Optimized for serum, plasma, urine and cell culture medium
    - 1/10th the sample volume of an ELISA

    Research Applications:
    - Detection of quantitative protein levels in biological fluids
    - Validation of antibody array results
    - Validation of biomarker discovery studies

    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed
    2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4 °C
    3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
    4. Add 15μL Primer solution to each well
    5. Add 10μL of PCR Master Mix to each well
    6. Run real-time PCR
    Préparation de l'échantillon

    Recommended Dilution (serum & plasma): 5 - 100 fold

    Précision du teste
    Serum spike tests show recovery is 92.5% with a range of 89.7 to 98.1%
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C,-80 °C
    Stockage commentaire
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8
    Date de péremption
    6 months
  • Antigène Voir toutes c-MET (MET) IQ-ELISA Kits
    c-MET (MET) (Met Proto-Oncogene (MET))
    Autre désignation
    HGFR (MET Produits)
    Synonymes
    AUTS9 Kit IQ-ELISA, HGFR Kit IQ-ELISA, RCCP2 Kit IQ-ELISA, c-Met Kit IQ-ELISA, AI838057 Kit IQ-ELISA, HGF Kit IQ-ELISA, Par4 Kit IQ-ELISA, Hgfr Kit IQ-ELISA, c-met Kit IQ-ELISA, MET Kit IQ-ELISA, C-MET Kit IQ-ELISA, met Kit IQ-ELISA, met-A Kit IQ-ELISA, MET proto-oncogene, receptor tyrosine kinase Kit IQ-ELISA, met proto-oncogene Kit IQ-ELISA, MET proto-oncogene, receptor tyrosine kinase L homeolog Kit IQ-ELISA, MET Kit IQ-ELISA, Met Kit IQ-ELISA, met.L Kit IQ-ELISA
    Sujet
    Synonyms: Hepatocyte growth factor receptor (HGF receptor) (EC 2.7.10.1) (HGF/SF receptor) (Proto-oncogene c-Met) (Scatter factor receptor) (SF receptor) (Tyrosine-protein kinase Met), MET
    ID gène
    4233
    UniProt
    P08581
    Pathways
    Signalisation RTK, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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