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PARP1 Kit ELISA
PARP1
Reactivité: Humain
Colorimetric
Cell ELISA
Adherent Cell Culture
N° du produit ABIN5690733
Détail du produit PARP1 Kit ELISA
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Antigène
Voir toutes PARP1 Kits ELISA
PARP1
(Poly (ADP-Ribose) Polymerase 1 (PARP1))
Reactivité
Toutes les réactivités sur PARP1 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Cell ELISA
Application
ELISA
Fonction
Human Cleaved PARP (D214/G215) Cell-based ELISA Kit. This ELISA is for measuring cleaved PARP (Asp-214/Gly-215) in human adherent cell lines.
Type d'échantillon
Adherent Cell Culture
Analytical Method
Semi-Quantitative
Specificité
The antibody pair provided in this kit recognizes human PARP cleaved at sites aspartic acid-214 and glycine-215.
Attributs du produit
Rapidly measure phosphorylated protein in adherent cell lines Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose) No sample lysis is needed Compatible with a standard ELISA plate reader
Ingrédients
Uncoated 96-well Strip Microplate Wash Buffers Fixing Solution Quenching Buffer Blocking Buffer Anti-phospho antibody Anti-pan antibody HRP-Conjugated Secondary Antibody TMB One-Step Substrate Stop Solution
Matériel non inclus
Distilled or deionized water 100 mL and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 μL to 1 mL volumes Adjustable 1-25 mL pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
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Alternatives
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Information d'application
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Plaque
Uncoated
Protocole
Prepare all reagents and samples as instructed in the manual. Add 100 μL of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
4 °C
Stockage commentaire
Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes PARP1 Kits ELISA
PARP1
(Poly (ADP-Ribose) Polymerase 1 (PARP1))
Autre désignation
PARP (PARP1 Produits )
Synonymes
ADPRT Kit ELISA, ADPRT 1 Kit ELISA, ADPRT1 Kit ELISA, ARTD1 Kit ELISA, PARP Kit ELISA, PARP-1 Kit ELISA, PPOL Kit ELISA, pADPRT-1 Kit ELISA, Adprt Kit ELISA, Parp-1 Kit ELISA, 5830444G22Rik Kit ELISA, AI893648 Kit ELISA, Adprp Kit ELISA, Adprt1 Kit ELISA, C80510 Kit ELISA, parp-1 Kit ELISA, sPARP-1 Kit ELISA, BEST:LD21673 Kit ELISA, CG17685 Kit ELISA, CG17696 Kit ELISA, CG17718 Kit ELISA, CG40411 Kit ELISA, D.PARP Kit ELISA, Dm.pARTa Kit ELISA, Dmel\\CG40411 Kit ELISA, LD21673.3prime Kit ELISA, PARP1 Kit ELISA, dPARP Kit ELISA, parp Kit ELISA, adprt1 Kit ELISA, padprt-1 Kit ELISA, ppol Kit ELISA, si:dkey-206f10.3 Kit ELISA, wu:fc60f12 Kit ELISA, zgc:110092 Kit ELISA, poly(ADP-ribose) polymerase 1 Kit ELISA, poly (ADP-ribose) polymerase 1 Kit ELISA, poly (ADP-ribose) polymerase family, member 1 Kit ELISA, Poly-(ADP-ribose) polymerase Kit ELISA, poly(ADP-ribose) polymerase 1 L homeolog Kit ELISA, PARP1 Kit ELISA, Parp1 Kit ELISA, Parp Kit ELISA, parp1.L Kit ELISA, parp1 Kit ELISA
ID gène
142
UniProt
P09874
Pathways
Apoptose , Caspase Cascade in Apoptosis , Réparation de l'ADN , Production of Molecular Mediator of Immune Response , Maintenance of Protein Location
Vus récemment
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