RPS6KA3 Kit ELISA

N° du produit ABIN5690735

Détail du produit RPS6KA3 Kit ELISA

Antigène: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Fonction: Human, Mouse and Rat Phospho-RSK2 (Ser386) and Total RSK2 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures RSK2 phosphorylated at Serine-386 as well as total RSK2 in adherent cell lines.

Type d'échantillon: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificité: The antibodies provided in this kit recognizes Human, Mouse and Rat RSK2 phosphorylated at site Serine-386 as well as total RSK2.

Attributs du produit:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Ingrédients:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Plaque: Uncoated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Autre désignation: RSK2 (RPS6KA3 Produits)

Synonymes: HU-1 Kit ELISA, MAPKAPK1A Kit ELISA, RSK Kit ELISA, RSK1 Kit ELISA, CLS Kit ELISA, HU-3 Kit ELISA, ISPK-1 Kit ELISA, MAPKAPK1B Kit ELISA, MRX19 Kit ELISA, RSK2 Kit ELISA, S6K-alpha3 Kit ELISA, p90-RSK2 Kit ELISA, pp90RSK2 Kit ELISA, MPK-9 Kit ELISA, Rsk2 Kit ELISA, RGD1563860 Kit ELISA, id:ibd1103 Kit ELISA, rps6ka3 Kit ELISA, si:ch211-114c12.12 Kit ELISA, wu:fk86e02 Kit ELISA, zgc:56006 Kit ELISA, zgc:158386 Kit ELISA, ribosomal protein S6 kinase A1 Kit ELISA, ribosomal protein S6 kinase A3 Kit ELISA, ribosomal protein S6 kinase polypeptide 3 Kit ELISA, ribosomal protein S6 kinase a, polypeptide 3a Kit ELISA, ribosomal protein S6 kinase, polypeptide 3b Kit ELISA, RPS6KA1 Kit ELISA, RPS6KA3 Kit ELISA, Rps6ka3 Kit ELISA, rps6ka3a Kit ELISA, rps6ka3b Kit ELISA

ID gène: 6197

UniProt: P51812, P18654, D3Z8E0

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades