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C-JUN Kit ELISA

JUN Reactivité: Humain Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
N° du produit ABIN5690749
  • Antigène Voir toutes C-JUN (JUN) Kits ELISA
    C-JUN (JUN) (Jun Proto-Oncogene (JUN))
    Reactivité
    • 23
    • 19
    • 17
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    DNA-Binding ELISA
    Application
    ELISA
    Fonction
    Human c-Jun Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical c-Jun binding sequences to semi-quantitatively detect active c-Jun in lysates or nuclear extracts.
    Type d'échantillon
    Cell Lysate, Nuclear Extract
    Analytical Method
    Semi-Quantitative
    Specificité
    The olionucleotide/antibody pair provided in this kit recognizes mouse c-Jun in whole lysates and nuclear extracts.
    Attributs du produit
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    Ingrédients
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Stockage commentaire
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Date de péremption
    6 months
  • Antigène Voir toutes C-JUN (JUN) Kits ELISA
    C-JUN (JUN) (Jun Proto-Oncogene (JUN))
    Autre désignation
    c-Jun (JUN Produits)
    Synonymes
    AP-1 Kit ELISA, AP1 Kit ELISA, c-Jun Kit ELISA, fj36h07 Kit ELISA, wu:fj36h07 Kit ELISA, zgc:65863 Kit ELISA, Junc Kit ELISA, c-jun Kit ELISA, CG2275 Kit ELISA, D-Jun Kit ELISA, D-jun Kit ELISA, D-jun/Jra Kit ELISA, DJUN Kit ELISA, DJun Kit ELISA, Djun Kit ELISA, Dmel\\CG2275 Kit ELISA, JRA Kit ELISA, JUN Kit ELISA, Jun Kit ELISA, V Kit ELISA, cJun Kit ELISA, d-JRA Kit ELISA, d-Jun Kit ELISA, d-jun Kit ELISA, dAP-1 Kit ELISA, dJRA Kit ELISA, dJUN Kit ELISA, dJra Kit ELISA, dJun Kit ELISA, djun Kit ELISA, dm-Jun Kit ELISA, jra Kit ELISA, jun Kit ELISA, l(2)46Ef Kit ELISA, l(2)IA109 Kit ELISA, l(2R)IA109 Kit ELISA, Jun proto-oncogene, AP-1 transcription factor subunit Kit ELISA, jun proto-oncogene S homeolog Kit ELISA, jun proto-oncogene Kit ELISA, Jun-related antigen Kit ELISA, jun proto-oncogene L homeolog Kit ELISA, JUN Kit ELISA, Jun Kit ELISA, jun.S Kit ELISA, jun Kit ELISA, Jra Kit ELISA, jun.L Kit ELISA
    ID gène
    3725
    UniProt
    P05412
    Pathways
    Signalisation MAPK, Signalisation RTK, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins
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