c-MYC Kit ELISA

N° du produit ABIN5690750

Détail du produit c-MYC Kit ELISA

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: Human c-Myc Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical c-Myc binding sequences to semi-quantitatively detect active c-Myc in lysates or nuclear extracts.

Type d'échantillon: Cell Lysate, Nuclear Extract

Analytical Method: Semi-Quantitative

Specificité: The olionucleotide/antibody pair provided in this kit recognizes human TFEB in whole lysates and nuclear extracts.

Attributs du produit:

• Specific transcription factor-DNA binding assay
• Perfect alternative to EMSA
• Easy to perform in an ELISA format
• Non-radioactive assay
• High throughput (96 well plate format)
• Assay can be completed within 5 hours

Ingrédients:

• 96-well Strip Microplate pre-coated with DNA probes
• DNA Binding Buffer
• Positive Control Sample
• Specific Competitor DNA probe
• Non-specific Competitor DNA probe
• Assay Reagent
• DTT
• Wash Buffer
• Primary Antibody
• HRP-conjugated Secondary Antibody
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Absorbent paper
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Autre désignation: c-Myc (MYC Produits)

Synonymes: MRTL Kit ELISA, MYCC Kit ELISA, bHLHe39 Kit ELISA, c-Myc Kit ELISA, CMYC Kit ELISA, c-myc Kit ELISA, C-MYC Kit ELISA, LOC100136746 Kit ELISA, ARC Kit ELISA, B430311C09Rik Kit ELISA, MYC Kit ELISA, NOP Kit ELISA, Nop30 Kit ELISA, RNCMYC Kit ELISA, mMyc Kit ELISA, AU016757 Kit ELISA, Myc2 Kit ELISA, Niard Kit ELISA, Nird Kit ELISA, cmyc Kit ELISA, zc-myc Kit ELISA, c-myc II Kit ELISA, myc-B Kit ELISA, myc2 Kit ELISA, myc1 Kit ELISA, CMyc Kit ELISA, zgc:55680 Kit ELISA, CG10798 Kit ELISA, D-Myc Kit ELISA, DM Kit ELISA, DMYc Kit ELISA, Dm Kit ELISA, Dmel\CG10798 Kit ELISA, Dmyc Kit ELISA, EG:BACN5I9.1 Kit ELISA, anon-WO03040301.171 Kit ELISA, bHLHe57 Kit ELISA, c-MYC Kit ELISA, d-myc Kit ELISA, dMYC Kit ELISA, dMyc Kit ELISA, dMyc1 Kit ELISA, da Kit ELISA, dm Kit ELISA, dm/dMyc Kit ELISA, dm/myc Kit ELISA, dmyc Kit ELISA, dmyc1 Kit ELISA, l(1)G0139 Kit ELISA, l(1)G0354 Kit ELISA, l(1)G0359 Kit ELISA, myc Kit ELISA, MYC proto-oncogene, bHLH transcription factor Kit ELISA, v-myc myelocytomatosis viral oncogene homolog Kit ELISA, nucleolar protein 3 (apoptosis repressor with CARD domain) Kit ELISA, myelocytomatosis oncogene Kit ELISA, MYC proto-oncogene, bHLH transcription factor a Kit ELISA, MYC proto-oncogene, bHLH transcription factor L homeolog Kit ELISA, MYC proto-oncogene, bHLH transcription factor S homeolog Kit ELISA, v-myc myelocytomatosis viral oncogene homolog (avian) Kit ELISA, MYC proto-oncogene, bHLH transcription factor b Kit ELISA, CG10798 gene product from transcript CG10798-RB Kit ELISA, MYC Kit ELISA, LOC100136746 Kit ELISA, Nol3 Kit ELISA, Myc Kit ELISA, myca Kit ELISA, myc.L Kit ELISA, myc.S Kit ELISA, mycb Kit ELISA

ID gène: 4609

UniProt: P01106

Pathways: Signalisation p53, Cycle Cellulaire, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, L'effet Warburg