Estrogen Receptor alpha Kit ELISA

N° du produit ABIN5690752

Détail du produit Estrogen Receptor alpha Kit ELISA

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: Human ER-alpha Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical ER-alpha binding sequences to semi-quantitatively detect active ER-alpha in lysates or nuclear extracts.

Type d'échantillon: Cell Lysate, Nuclear Extract

Analytical Method: Semi-Quantitative

Specificité: The olionucleotide/antibody pair provided in this kit recognizes mouse ER-alpha in whole lysates and nuclear extracts.

Attributs du produit:

• Specific transcription factor-DNA binding assay
• Perfect alternative to EMSA
• Easy to perform in an ELISA format
• Non-radioactive assay
• High throughput (96 well plate format)
• Assay can be completed within 5 hours

Ingrédients:

• 96-well Strip Microplate pre-coated with DNA probes
• DNA Binding Buffer
• Positive Control Sample
• Specific Competitor DNA probe
• Non-specific Competitor DNA probe
• Assay Reagent
• DTT
• Wash Buffer
• Primary Antibody
• HRP-conjugated Secondary Antibody
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions Absorbent paper
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Autre désignation: ER-alpha (ESR1 Produits)

Synonymes: LOC398734 Kit ELISA, AA420328 Kit ELISA, AU041214 Kit ELISA, ER-alpha Kit ELISA, ER[a] Kit ELISA, ERa Kit ELISA, ERalpha Kit ELISA, ESR Kit ELISA, Estr Kit ELISA, Estra Kit ELISA, Nr3a1 Kit ELISA, NR3A1 Kit ELISA, eralpha Kit ELISA, zfER[a] Kit ELISA, ER Kit ELISA, ESRA Kit ELISA, ESTRR Kit ELISA, Era Kit ELISA, akap12 Kit ELISA, cb27 Kit ELISA, esr1 Kit ELISA, fb72g12 Kit ELISA, id:ibd1202 Kit ELISA, sb:cb27 Kit ELISA, si:ch73-192g21.1 Kit ELISA, wu:fb72g12 Kit ELISA, wu:fc21c03 Kit ELISA, Esr Kit ELISA, RNESTROR Kit ELISA, ERALPHA Kit ELISA, er Kit ELISA, esr Kit ELISA, nr3a1 Kit ELISA, XER Kit ELISA, era Kit ELISA, esra Kit ELISA, ERbeta Kit ELISA, xesr-1 Kit ELISA, xlERalpha1 Kit ELISA, xlERalpha2 Kit ELISA, ESR1 Kit ELISA, estrogen receptor 1 L homeolog Kit ELISA, estrogen receptor 1 (alpha) Kit ELISA, estrogen receptor 1 Kit ELISA, A kinase (PRKA) anchor protein 12b Kit ELISA, esr1.L Kit ELISA, Esr1 Kit ELISA, ESR1 Kit ELISA, esr1 Kit ELISA, akap12b Kit ELISA

ID gène: 2099

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly