PPARG Kit ELISA

N° du produit ABIN5690762

Détail du produit PPARG Kit ELISA

Antigène: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction:  Human PPAR-gamma Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical PPAR-gamma binding sequences to semi-quantitatively detect active PPAR-gamma in lysates or nuclear extracts. 

Type d'échantillon: Cell Lysate, Nuclear Extract

Analytical Method: Semi-Quantitative

Specificité: The olionucleotide/antibody pair provided in this kit recognizes human PPAR-gamma in whole lysates and nuclear extracts.

Attributs du produit:

• Specific transcription factor-DNA binding assay
• Perfect alternative to EMSA
• Easy to perform in an ELISA format
• Non-radioactive assay
• High throughput (96 well plate format)
• Assay can be completed within 5 hours

Ingrédients:

• 96-well Strip Microplate pre-coated with DNA probes
• DNA Binding Buffer
• Positive Control Sample
• Specific Competitor DNA probe
• Non-specific Competitor DNA probe
• Assay Reagent
• DTT
• Wash Buffer
• Primary Antibody
• HRP-conjugated Secondary Antibody
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions Absorbent paper
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Autre désignation: PPAR-gamma (PPARG Produits)

Synonymes: PPAR gamma Kit ELISA, PPARg2 Kit ELISA, PPARG Kit ELISA, Nr1c3 Kit ELISA, PPAR-gamma Kit ELISA, PPAR-gamma2 Kit ELISA, PPARgamma Kit ELISA, PPARgamma2 Kit ELISA, NR1C3 Kit ELISA, CIMT1 Kit ELISA, GLM1 Kit ELISA, PPARG1 Kit ELISA, PPARG2 Kit ELISA, xPPAR-gamma Kit ELISA, PPAR-GAMMA Kit ELISA, PPARGAMMA Kit ELISA, peroxisome proliferator-activated receptor gamma Kit ELISA, peroxisome proliferator activated receptor gamma Kit ELISA, peroxisome proliferator activated receptor gamma L homeolog Kit ELISA, PPARG Kit ELISA, Pparg Kit ELISA, pparg.L Kit ELISA

ID gène: 5468

UniProt: P37231

Pathways: Signalisation MAPK, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation