CER1 Kit ELISA

N° du produit ABIN5690782

Détail du produit CER1 Kit ELISA

Antigène: CER1 (Cerberus 1 Homolog (Xenopus Laevis) (CER1))

Reactivité: Poulet

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Chicken Caronte ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Chicken Caronte.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: CER1 (Cerberus 1 Homolog (Xenopus Laevis) (CER1))

Autre désignation: Caronte (CER1 Produits)

Synonymes: DAND4 Kit ELISA, CAR Kit ELISA, cer Kit ELISA, cer-1 Kit ELISA, dand4 Kit ELISA, tCerberus Kit ELISA, xcer-1 Kit ELISA, xcer1 Kit ELISA, Cerl Kit ELISA, Cerl1 Kit ELISA, Cerr1 Kit ELISA, RGD1563046 Kit ELISA, cerberus 1, DAN family BMP antagonist Kit ELISA, cerberus 1, DAN family BMP antagonist S homeolog Kit ELISA, CER1 Kit ELISA, cer1.S Kit ELISA, Cer1 Kit ELISA

ID gène: 395623

UniProt: Q9PUK2

Pathways: Maintenance of Protein Location