ACPP Kit ELISA
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- Antigène Voir toutes ACPP Kits ELISA
- ACPP (Acid Phosphatase, Prostate (ACPP))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human ACPP ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Human ACPP.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 6 months
-
- Antigène Voir toutes ACPP Kits ELISA
- ACPP (Acid Phosphatase, Prostate (ACPP))
- Autre désignation
- ACPP (ACPP Produits)
- Synonymes
- 5'-NT Kit ELISA, ACP-3 Kit ELISA, ACP3 Kit ELISA, PAP Kit ELISA, AMAP2 Kit ELISA, CENTB3 Kit ELISA, DDEF2 Kit ELISA, PAG3 Kit ELISA, Pap-alpha Kit ELISA, SHAG1 Kit ELISA, A030005E02Rik Kit ELISA, FRAP Kit ELISA, Lap Kit ELISA, Ppal Kit ELISA, Acpp11 Kit ELISA, RNACPP11 Kit ELISA, pap Kit ELISA, ACPP Kit ELISA, acp-3 Kit ELISA, acp3 Kit ELISA, acpt Kit ELISA, acid phosphatase, prostate Kit ELISA, ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 Kit ELISA, prostatic acid phosphatase Kit ELISA, prostatic acid phosphatase, putative Kit ELISA, acid phosphatase, prostate S homeolog Kit ELISA, ACPP Kit ELISA, ASAP2 Kit ELISA, Acpp Kit ELISA, CpipJ_CPIJ004002 Kit ELISA, Smp_016640 Kit ELISA, acpp.S Kit ELISA
- ID gène
- 55
- UniProt
- P15309
- Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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