Caspase 1 Kit ELISA

N° du produit ABIN5690837

Détail du produit Caspase 1 Kit ELISA

Antigène: Caspase 1 (CASP1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Caspase-1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Caspase-1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: Caspase 1 (CASP1)

Autre désignation: Caspase-1 (CASP1 Produits)

Synonymes: ICE Kit ELISA, IL1BC Kit ELISA, P45 Kit ELISA, Il1bc Kit ELISA, Ice Kit ELISA, CASP1 Kit ELISA, CG5370 Kit ELISA, DCP-1 Kit ELISA, DCP1 Kit ELISA, Dcp1 Kit ELISA, Dmel\\CG5370 Kit ELISA, ccp1 Kit ELISA, dcp-1 Kit ELISA, dcp1 Kit ELISA, l(2)01862 Kit ELISA, l(2)02132 Kit ELISA, caspase 1 Kit ELISA, caspase 1, apoptosis-related cysteine peptidase Kit ELISA, caspase 4, apoptosis-related cysteine peptidase Kit ELISA, Death caspase-1 Kit ELISA, caspase-1 Kit ELISA, caspase Kit ELISA, caspase recruitment domain-containing protein 16 Kit ELISA, caspase 4 Kit ELISA, CASP1 Kit ELISA, Casp1 Kit ELISA, CASP4 Kit ELISA, Dcp-1 Kit ELISA, Caspase1 Kit ELISA, LOC5564196 Kit ELISA, LOC451519 Kit ELISA

ID gène: 834

UniProt: P29466

Pathways: Apoptose, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome