Crk Kit ELISA

N° du produit ABIN5690859

Détail du produit Crk Kit ELISA

Antigène: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Crk ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Crk.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Autre désignation: Crk (CRK Produits)

Synonymes: CG1587 Kit ELISA, CRK Kit ELISA, D-CRK Kit ELISA, D-Crk Kit ELISA, DCrk Kit ELISA, Dcrk Kit ELISA, Dmel\\CG1587 Kit ELISA, crk Kit ELISA, dCRK Kit ELISA, dCrk Kit ELISA, fb34h10 Kit ELISA, fc05a01 Kit ELISA, fi19g05 Kit ELISA, wu:fb34h10 Kit ELISA, wu:fc05a01 Kit ELISA, wu:fc54a04 Kit ELISA, wu:fi19g05 Kit ELISA, zgc:100936 Kit ELISA, CRKII Kit ELISA, p38 Kit ELISA, Crko Kit ELISA, crk2 Kit ELISA, crkII Kit ELISA, v-crk Kit ELISA, P38C-CRK Kit ELISA, Crk-I Kit ELISA, Crk-II Kit ELISA, Crk-III Kit ELISA, Crk3 Kit ELISA, CrkIII Kit ELISA, c-Crk Kit ELISA, Crk oncogene Kit ELISA, v-crk avian sarcoma virus CT10 oncogene homolog Kit ELISA, CRK proto-oncogene, adaptor protein Kit ELISA, v-crk avian sarcoma virus CT10 oncogene homolog L homeolog Kit ELISA, v-crk sarcoma virus CT10 oncogene homolog Kit ELISA, Crk Kit ELISA, crk Kit ELISA, CRK Kit ELISA, crk.L Kit ELISA

ID gène: 1398

UniProt: P46108

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor