Cyclin D1 Kit ELISA

N° du produit ABIN5690860

Détail du produit Cyclin D1 Kit ELISA

Antigène: Cyclin D1 (CCND1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Cyclin D1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Cyclin D1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: Cyclin D1 (CCND1)

Autre désignation: Cyclin D1 (CCND1 Produits)

Synonymes: BCL1 Kit ELISA, D11S287E Kit ELISA, PRAD1 Kit ELISA, U21B31 Kit ELISA, AI327039 Kit ELISA, CycD1 Kit ELISA, Cyl-1 Kit ELISA, bcl-1 Kit ELISA, cD1 Kit ELISA, cb161 Kit ELISA, cycd1 Kit ELISA, etID37810.7 Kit ELISA, fb52e01 Kit ELISA, fc45c08 Kit ELISA, fc83a12 Kit ELISA, id:ibd1198 Kit ELISA, wu:fb52e01 Kit ELISA, wu:fc45c08 Kit ELISA, wu:fc83a12 Kit ELISA, ccnd1b Kit ELISA, prad1 Kit ELISA, ccnd1 Kit ELISA, ccnd1a Kit ELISA, bcl1 Kit ELISA, d11s287e Kit ELISA, u21b31 Kit ELISA, CCND1 Kit ELISA, cyclin D1 Kit ELISA, cyclin D1 S homeolog Kit ELISA, cyclin D1 L homeolog Kit ELISA, CCND1 Kit ELISA, Ccnd1 Kit ELISA, ccnd1 Kit ELISA, ccnd1.S Kit ELISA, ccnd1.L Kit ELISA

ID gène: 595

UniProt: P24385

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling