Dihydrofolate Reductase Kit ELISA

N° du produit ABIN5690864

Détail du produit Dihydrofolate Reductase Kit ELISA

Antigène: Dihydrofolate Reductase (DHFR)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human DHFR ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human DHFR.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: Dihydrofolate Reductase (DHFR)

Autre désignation: DHFR (DHFR Produits)

Synonymes: DHFR Kit ELISA, F10_orf160 Kit ELISA, ECK0049 Kit ELISA, JW0047 Kit ELISA, tmrA Kit ELISA, cb595 Kit ELISA, zgc:86637 Kit ELISA, DDBDRAFT_0187132 Kit ELISA, DDBDRAFT_0230113 Kit ELISA, DDB_0187132 Kit ELISA, DDB_0230113 Kit ELISA, CG14887 Kit ELISA, Dmel\\CG14887 Kit ELISA, dhfr Kit ELISA, DHFRP1 Kit ELISA, DYR Kit ELISA, 8430436I03Rik Kit ELISA, AA607882 Kit ELISA, AI662710 Kit ELISA, AW555094 Kit ELISA, Dhfr1 Kit ELISA, confers resistance to sulfonamides Kit ELISA, dihydrofolate reductase Kit ELISA, dihydrofolate reductase DhfR Kit ELISA, Dihydrofolate reductase Kit ELISA, dhfr Kit ELISA, DHFR Kit ELISA, dhfR Kit ELISA, folA Kit ELISA, dfrA Kit ELISA, LOC707382 Kit ELISA, CNJ01940 Kit ELISA, DDB_G0286755 Kit ELISA, Dhfr Kit ELISA, Mesil_0096 Kit ELISA, Arch_0408 Kit ELISA, Fbal_3367 Kit ELISA, Ilyop_0482 Kit ELISA, Lbys_2007 Kit ELISA, Riean_0733 Kit ELISA, Mrub_1855 Kit ELISA, Poras_0766 Kit ELISA, Halhy_3552 Kit ELISA, Mesop_3614 Kit ELISA, LOC609048 Kit ELISA

ID gène: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases