ELF3 Kit ELISA
ELF3
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690871
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Antigène
Tous les produits ELF3
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ELF3
(E74-Like Factor 3 (Ets Domain Transcription Factor, Epithelial-Specific) (ELF3))
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Reactivité
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Humain
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Human ELF3 ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Specificité
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This ELISA antibody pair recognizes Human ELF3.
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Stock
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4 °C
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Date de péremption
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6 months
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Antigène
Tous les produits ELF3
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ELF3
(E74-Like Factor 3 (Ets Domain Transcription Factor, Epithelial-Specific) (ELF3))
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Autre désignation
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ELF3 (ELF3 Produits)
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Synonymes
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ert Kit ELISA, esx Kit ELISA, epr-1 Kit ELISA, ese-1 Kit ELISA, ESE-1 Kit ELISA, ESX Kit ELISA, jen Kit ELISA, EPR-1 Kit ELISA, ERT Kit ELISA, E74 like ETS transcription factor 3 Kit ELISA, E74-like factor 3 Kit ELISA, elf3 Kit ELISA, Elf3 Kit ELISA, ELF3 Kit ELISA
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ID gène
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1999
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UniProt
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P78545
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