FOXO3 Kit ELISA

N° du produit ABIN5690877

Détail du produit FOXO3 Kit ELISA

Antigène: FOXO3 (Forkhead Box O3 (FOXO3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human FoxO3/FKHRL1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human FoxO3.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: FOXO3 (Forkhead Box O3 (FOXO3))

Autre désignation: FoxO3 (FKHRL1 (FOXO3 Produits)

Synonymes: AF6q21 Kit ELISA, FKHRL1 Kit ELISA, FKHRL1P2 Kit ELISA, FOXO2 Kit ELISA, FOXO3A Kit ELISA, zgc:92176 Kit ELISA, wu:fb50g02 Kit ELISA, wu:fi33f07 Kit ELISA, Fkhrl1 Kit ELISA, Foxo3a Kit ELISA, FoxO3A Kit ELISA, 1110048B16Rik Kit ELISA, 2010203A17Rik Kit ELISA, C76856 Kit ELISA, Fkhr2 Kit ELISA, FOXO-3 Kit ELISA, FOXO3 Kit ELISA, foxo3a Kit ELISA, xfoxo3 Kit ELISA, foxO3 Kit ELISA, foxO Kit ELISA, fkhr Kit ELISA, foxo5 Kit ELISA, wu:fj65a12 Kit ELISA, zFkhr Kit ELISA, forkhead box O3 Kit ELISA, forkhead box O3A Kit ELISA, forkhead box O3 L homeolog Kit ELISA, forkhead box O3b Kit ELISA, FOXO3 Kit ELISA, foxo3a Kit ELISA, Foxo3 Kit ELISA, foxo3.L Kit ELISA, foxo3 Kit ELISA, foxO3 Kit ELISA, foxo3b Kit ELISA

ID gène: 2309

UniProt: O43524

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis